Biochemical and Biophysical Research Communications 2015-01-01

Inhibition of GDP beta S of agonist-activated phospholipase C in human platelets requires cell permeabilization.

Liting Guo, Haijun Zhang, Fei Wang, Ping Liu, Yonglu Wang, Guohua Xia, Ran Liu, Xueming Li, Haixiang Yin, Hulin Jiang, Baoan Chen

文献索引：Biochem. Biophys. Res. Commun. 153 , 417-421, (1988)

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摘要

The inhibition by guanosine 5'-[beta-thio]diphosphate (GDP beta S) of phospholipase C was compared in intact and saponin-permeabilized human platelets in order to assess whether effects of GDP beta S on phospholipase C activation unrelated to guanine nucleotide binding function were occurring. GDP beta S exhibited no effect on phospholipase C activity, monitored by phosphatidic acid formation, in intact platelets that were unstimulated or stimulated with 0.5 U/ml thrombin or 20 nM ONO-11113 (a stable thromboxane A2 analogue). However, GDP beta S did cause a marked decrease in the activity of phospholipase C in saponin-permeabilized platelets. Thus GDP beta S is a viable tool for studying the role of G-proteins in transducing receptor-mediated activation of phospholipase C in platelets.