Biotechnology Progress 2012-01-01

Biotransformation of 2,6-diaminopurine nucleosides by immobilized Geobacillus stearothermophilus.

Eliana C De Benedetti, Cintia W Rivero, Claudia N Britos, Mario E Lozano, Jorge A Trelles

文献索引：Biotechnol. Prog. 28(5) , 1251-6, (2012)

全文：HTML全文

摘要

An efficient and green bioprocess to obtain 2,6-diaminopurine nucleosides using thermophilic bacteria is herein reported. Geobacillus stearothermophilus CECT 43 showed a conversion rate of 90 and 83% at 2 h to obtain 2,6-diaminopurine-2'-deoxyriboside and 2,6-diaminopurine riboside, respectively. The selected biocatalyst was successfully stabilized in an agarose matrix and used to produce up to 23.4 g of 2,6-diaminopurine-2'-deoxyriboside in 240 h of process. These nucleoside analogues can be used as prodrug precursors or in antisense oligonucleotide synthesis.Copyright © 2012 American Institute of Chemical Engineers (AIChE).