PLoS ONE 2014-01-01

Ultrasensitive electrochemical immunoassay for avian influenza subtype H5 using nanocomposite.

Zhixun Xie, Jiaoling Huang, Sisi Luo, Zhiqin Xie, Liji Xie, Jiabo Liu, Yaoshan Pang, Xianwen Deng, Qing Fan

文献索引：PLoS ONE 9(4) , e94685, (2014)

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摘要

We report a novel electrochemical immunosensor that can sensitively detect avian influenza virus H5 subtype (AIV H5) captured by graphene oxide-H5-polychonal antibodies-bovine serum albumin (GO-PAb-BSA) nanocomposite. The graphene oxide (GO) carried H5-polychonal antibody (PAb) were used as signal amplification materials. Upon signal amplification, the immunosensor showed a 256-fold increase in detection sensitivity compared to the immunosensor without GO-PAb-BSA. We designed a PAb labeling GO strategy and signal amplification procedure that allow ultrasensitive and selective detection of AIV H5. The established method responded to 2(-15) HA unit/50 µL H5, with a linear calibration range from 2(-15) to 2(-8) HA unit/50 µL. In summary, we demonstrated that the immunosenser has a high specificity and sensitivity for AIV H5, and the established assay could be potentially applied in the rapid detection of other pathogenic microorganisms.