Abstract Equations relating the N-acylation rate constants of glycine, L-α-alanine, DL- threonine, and L-proline with 4-nitrophenyl 4-nitrobenzoate in water-acetonitrile, water-2- propanol, and water-2-methyl-2-propanol solvents to the composition of the medium were obtained, and reaction rate constants in water were calculated.