Isoprenoid biosynthesis in Escherichiacoli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a …
A crude cell-free system from an Escherichiacoli strain overexpressing the cluster containing the three genes yfgA, yfgB, and gcpE converted 2-C-methyl-d-erythritol 2, 4- cyclodiphosphate (1) into a phosphorylated derivative of (E)-2-methylbut-2-ene-1, 4-diol (6), which most probably represents a novel intermediate in the methylerythritol phosphate pathway for isoprenoid biosynthesis. The free diol 6 was accumulated by phosphatase ...
