Tetrahedron letters

Isoprenoid biosynthesis in Escherichiacoli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a …

M Seemann, N Campos, M Rodriguez-Concepción…

Index: Seemann, Myriam; Campos, Narciso; Rodriguez-Concepcion, Manuel; Ibanez, Ester; Duvold, Tore; Tritsch, Denis; Boronat, Albert; Rohmer, Michel Tetrahedron Letters, 2002 , vol. 43, # 8 p. 1413 - 1415

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Citation Number: 44

Abstract

A crude cell-free system from an Escherichiacoli strain overexpressing the cluster containing the three genes yfgA, yfgB, and gcpE converted 2-C-methyl-d-erythritol 2, 4- cyclodiphosphate (1) into a phosphorylated derivative of (E)-2-methylbut-2-ene-1, 4-diol (6), which most probably represents a novel intermediate in the methylerythritol phosphate pathway for isoprenoid biosynthesis. The free diol 6 was accumulated by phosphatase ...