Bioorganic & medicinal chemistry

New, stronger nucleophiles for nucleic acid-templated chemistry: Synthesis and application in fluorescence detection of cellular RNA

GP Miller, AP Silverman, ET Kool

文献索引：Miller, Gregory P.; Silverman, Adam P.; Kool, Eric T. Bioorganic and Medicinal Chemistry, 2008 , vol. 16, # 1 p. 56 - 64

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被引用次数: 30

摘要

Nucleic acid-templated chemistry is a promising strategy for imaging genetic sequences in living cells. Here we describe the synthesis of two new nucleophiles for use in templated nucleophilic displacements with DNA probes. The nucleophilic groups are phosphorodithioate and phosphorotrithioate; we report on synthetic methods for introducing these groups at the 3′-terminus of oligonucleotides. Both new nucleophiles are found to ...