Active-site-directed inhibition of. alpha.-chymotrypsin by deaminatively produced carbonium ions: an example of suicide or enzyme-activated-substrate inhibition

…, LW Jelinski, IR Politzer, BR Branchini…

文献索引：White, Emil H.; Jelinski, Lynn W.; Politzer, Ieva R.; Branchini, Bruce R.; Roswell, David F. Journal of the American Chemical Society, 1981 , vol. 103, # 14 p. 4231 - 4239

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被引用次数: 28

摘要

Abstract: N-Nitroso amides derived from phenylalanine and alanine were utilized as inhibitors of a-chymotrypsin, During the enzyme-catalyzed hydrolysis of these substrates, carbonium ions capable of alkylating nucleophilic groups are released in the active site. Nitroso lactams were also tested as substrates since they produce carbonium ions while still tethered to the enzyme at the acyl-enzyme stage. Kinetic studies indicated that at ...