A comparison of flexible and constrained haptens in eliciting antibody catalysts for paraoxon hydrolysis
DA Spivak, TZ Hoffman, AH Moore, MJ Taylor…
文献索引：Spivak, David A.; Hoffman, Timothy Z.; Moore, Alisa H.; Taylor, Matthew J.; Janda, Kim D. Bioorganic and Medicinal Chemistry, 1999 , vol. 7, # 6 p. 1145 - 1150
A new amine-oxide hapten was employed as an antigen, producing seven monoclonal antibodies (mAbs) from a panel of 20 that catalyzed paraoxon hydrolysis. The current hapten design differs from that previously described in that the molecule is inherently more flexible than its constrained predecessor. One of the seven antibody catalysts, mAb 1H9, showed the highest activity and was selected for detailed study. At pH= 8.77, the catalytic ...
