Facile synthesis of oligodeoxyribonucleotides via the phosphoramidite method without nucleoside base protection

Y Hayakawa, M Kataoka

文献索引：Hayakawa, Yoshihiro; Kataoka, Masanori Journal of the American Chemical Society, 1998 , vol. 120, # 48 p. 12395 - 12401

全文：HTML全文

被引用次数: 78

摘要

A facile synthesis of oligodeoxyribonucleotides via the phosphoramidite approach without base protection of the building blocks has been developed; it relies on the use of imidazolium triflate as a promoter for the condensation of a nucleoside phosphoramidite and a nucleoside. In the solution phase, the condensation is accomplished in a highly O- selective manner by using equimolar amounts of an N-free nucleoside phosphoramidite ...