Unexpected preference of the E. coli translation system for the ester bond during incorporation of backbone-elongated substrates

…, K Abe, N Sato, T Shibata, K Mizusawa…

文献索引：Sando, Shinsuke; Abe, Kenji; Sato, Nobuhiko; Shibata, Toshihiro; Mizusawa, Keigo; Aoyama, Yasuhiro Journal of the American Chemical Society, 2007 , vol. 129, # 19 p. 6180 - 6186

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被引用次数: 13

摘要

There have been recent advances in the ribosomal synthesis of various molecules composed of nonnatural ribosomal substrates. However, the ribosome has strict limitations on substrates with elongated backbones. Here, we show an unexpected loophole in the E. coli translation system, based on a remarkable disparity in its selectivity for β-amino/hydroxy acids. We challenged β-hydroxypropionic acid (β-HPA), which is less nucleophilic than β- ...