Efficient preparation of natural and synthetic galactosides with a recombinant β-1, 4-galactosyltransferase-/UDP-4'-gal epimerase fusion protein

O Blixt, J Brown, MJ Schur, W Wakarchuk…

文献索引：Blixt; Brown; Schur; Wakarchuk; Paulson Journal of Organic Chemistry, 2001 , vol. 66, # 7 p. 2442 - 2448

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被引用次数: 68

摘要

The numerous biological roles of LacNAc-based oligosaccharides have led to an increased demand for these structures for biological studies. In this report, an efficient route for the synthesis of β-galactosides using a bacterial β-4-galactosyltransferase/-UDP-4'-gal- epimerase fusion protein is described. The lgtB gene from Neisseria meningitidis and the galE gene from Streptococcus thermophilus were fused and cloned into an expression ...