A novel enantioselective epoxide hydrolase from Agromyces mediolanus ZJB120203: Cloning, characterization and application

F Xue, ZQ Liu, SP Zou, NW Wan, WY Zhu, Q Zhu…

文献索引：Xue, Feng; Liu, Zhi-Qiang; Zou, Shu-Ping; Wan, Nan-Wei; Zhu, Wen-Yuan; Zhu, Qing; Zheng, Yu-Guo Process Biochemistry, 2014 , vol. 49, # 3 p. 409 - 417

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被引用次数: 8

摘要

Abstract A new strain Agromyces mediolanus ZJB120203, capable of enantioselective epoxide hydrolase (EH) activity was isolated employing a newly established colorimetric screening and chiral GC analysis method. The partial nucleotide sequence of an epoxide hydrolase (AmEH) gene from A. mediolanus ZJB120203 was obtained by PCR using degenerate primers designed based on the conserved domains of EHs. Subsequently, an ...