Conscripting the Active-Site Zinc Ion in Carboxypeptidase A in Inactivation Chemistry by a New Type of Irreversible Enzyme Inactivator

Y Tanaka, I Grapsas, S Dakoji, YJ Cho…

文献索引：Tanaka; Grapsas; Dakoji; Cho; Mobashery Journal of the American Chemical Society, 1994 , vol. 116, # 17 p. 7475 - 7480

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被引用次数: 31

摘要

Abstract: A new strategy for irreversible inactivation of the metalloenzyme carboxypeptidase A (CPA) involving a proposed activation of a carbon-iodide bond in an inactivator by the active-site zinc ion toward nucleophilic substitution is described. 2-Benzyl-3-iodopropanoic acid (compound 1) was designed to bind the active site of CPA. An energyminimized complex of 1 in the active site of CPA reveals that the iodo moiety comes within the ...