Substrate induced pK perturbations with chymotrypsin and the possible significance of nonproductive binding

B Zeeberg, M Caplow, M Caswell

文献索引：Zeeberg; Caplow; Caswell Journal of the American Chemical Society, 1975 , vol. 97, # 25 p. 7346 - 7352

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被引用次数: 6

摘要

Abstract: The binding of the substrates N-acetyl-L-tyrosine p-chloroanilide and N-formyl-L- phenylalanine formylhydrazide to chymotrypsin is accompanied by a significant perturbation of the pK of a group on the enzyme. This perturbation does not occur with the closely related nonsubstrate derivatives N-formyl-D-phenylalanine formylhydrazide, N-formyl-N-methyl-L- phenylalanine formylhydrazide, N-acetyl-D-tyrosine p-chloroanilide, and N-acetyl-N- ...