Biosynthetic gene-based secondary metabolite screening: a new diterpene, methyl phomopsenonate, from the fungus Phomopsis amygdali

…, T Tokiwano, Y Kanno, Y Kanno, R Niida…

文献索引：Toyomasu, Tomonobu; Kaneko, Akane; Tokiwano, Tetsuo; Kanno, Yuya; Kanno, Yuri; Niida, Rie; Miura, Shigeyoshi; Nishioka, Taiki; Ikeda, Chiho; Mitsuhashi, Wataru; Dairi, Tohru; Kawano, Tomikazu; Oikawa, Hideaki; Kato, Nobuo; Sassa, Takeshi Journal of Organic Chemistry, 2009 , vol. 74, # 4 p. 1541 - 1548

全文：HTML全文

被引用次数: 25

摘要

The presence of the geranylgeranyl diphosphate synthase (GGS) gene is a common feature of gene clusters for diterpene biosynthesis. We demonstrated identification of a diterpene gene cluster using homology-based PCR of GGS genes and the subsequent genome walking in the fungus Phomopsis amygdali N2. Structure determination of a novel diterpene hydrocarbon phomopsene provided by enzymatic synthesis with the recombinant terpene ...