Analytical chemistry

Strategy for the isolation, derivatization, chromatographic separation, and detection of carnitine and acylcarnitines

PE Minkler, ST Ingalls, CL Hoppel

文献索引：Minkler, Paul E.; Ingalls, Stephen T.; Hoppel, Charles L. Analytical Chemistry, 2005 , vol. 77, # 5 p. 1448 - 1457

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被引用次数: 46

摘要

A strategy for detection of carnitine and acylcarnitines is introduced. This versatile system has four components:(1) isolation by protein precipitation/desalting and cation-exchange solid-phase extraction,(2) derivatization of carnitine and acylcarnitines with pentafluorophenacyl trifluoromethanesulfonate,(3) sequential ion-exchange/reversed-phase chromatography using a single non-end-capped C8 column, and (4) detection of carnitine ...