A strategy for detection of carnitine and acylcarnitines is introduced. This versatile system has four components:(1) isolation by protein precipitation/desalting and cation-exchange solid-phase extraction,(2) derivatization of carnitine and acylcarnitines with pentafluorophenacyl trifluoromethanesulfonate,(3) sequential ion-exchange/reversed-phase chromatography using a single non-end-capped C8 column, and (4) detection of carnitine ...
