Nitrile reductase from Geobacillus kaustophilus: a potential catalyst for a new nitrile biotransformation reaction

B Wilding, M Winkler, B Petschacher…

文献索引：Wilding, Birgit; Winkler, Margit; Petschacher, Barbara; Kratzer, Regina; Glieder, Anton; Klempier, Norbert Advanced Synthesis and Catalysis, 2012 , vol. 354, # 11-12 p. 2191 - 2198

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被引用次数: 21

摘要

Abstract The cloning, expression and characterization of a nitrile reductase (NRed) from the thermophile Geobacillus kaustophilus is reported. The enzyme shows a 12-fold increase in activity in response to a temperature change from 25 C to 65 C. The substrate scope regarding its biocatalytic applicability was investigated by testing a range of common nitriles. The narrow substrate range observed for the wild-type enzyme prompted the rational ...