A flexizyme that selectively charges amino acids activated by a water-friendly leaving group

N Niwa, Y Yamagishi, H Murakami, H Suga

文献索引：Niwa, Nobuyoshi; Yamagishi, Yusuke; Murakami, Hiroshi; Suga, Hiroaki Bioorganic and Medicinal Chemistry Letters, 2009 , vol. 19, # 14 p. 3892 - 3894

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被引用次数: 37

摘要

We have developed a new flexizyme (a flexible de novo tRNA acylation ribozyme) system, a pair of amino-derivatized benzyl thioester (ABT) and amino flexizyme (aFx). ABT bearing the ammonium ion was designed to render the acyl-donor substrates better water solubility. Although the previously reported flexizymes (eFx and dFx) did not show acylation activity for the ABT derivatives, a new flexizyme variant aFx, generated by in vitro selection against ...