Reliable chemical synthesis of oligoribonucleotides (RNA) with 2′??O??[(triisopropylsilyl) oxy] methyl (2′??O??tom)??protected phosphoramidites

S Pitsch, PA Weiss, L Jenny, A Stutz…

文献索引：Pitsch, Stefan; Weiss, Patrick A.; Jenny, Luzi; Stutz, Alfred; Wu, Xiaolin Helvetica Chimica Acta, 2001 , vol. 84, # 12 p. 3773 - 3795

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被引用次数: 209

摘要

Abstract A method for the introduction of the 2′-O-[(triisopropylsilyl) oxy] methyl (= tom) group into N-acetylated, 5′-O-dimethoxytritylated ribonucleosides is presented. The corresponding 2′-O-tom-protected phosphoramidite building blocks were obtained in pure form and were successfully employed for the routine synthesis of oligoribonucleotides on DNA synthesizers. Under DNA coupling conditions (2.5 min coupling time for a 1.5-μmol ...