Synthesis of fluorophore and quencher monomers for use in Scorpion primers and nucleic acid structural probes

CM McKeen, LJ Brown, JTG Nicol, JM Mellor…

文献索引：McKeen, Catherine M.; Brown, Lynda J.; Nicol, Jamie T.G.; Mellor, John M.; Brown, Tom Organic and Biomolecular Chemistry, 2003 , vol. 1, # 13 p. 2267 - 2275

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被引用次数: 43

摘要

We report the syntheses of monomers to incorporate fluorescein and methyl red within the sequence and at the termini of modified oligonucleotides. These monomers have been used in the solid-phase synthesis of fluorogenic oligonucleotides for genetic analysis and the study of multi-stranded nucleic acid structures.