... Chem., 52 (9), 2367-2368, 1988 2367 ... But this chemical method has the following twodisadvantages ... 20g glycerol, 5g peptone, 5g yeast extract, lg KH2PO4, 3g KH2PO4, 0.5g MgSO4-7H2O, 5gNaCl, 0.01 g FeSO4-7H2O, 0.01 g MnSO4-4H2O and 2g dl-ATC in a total ...