Synthesis and characterization of CN-modified protein analogues as potential vibrational contrast agents

M Noestheden, Q Hu, LL Tay, AM Tonary, A Stolow…

文献索引：Noestheden, Matthew; Hu, Qingyan; Tay, Li-Lin; Tonary, Angela M.; Stolow, Albert; MacKenzie, Roger; Tanha, Jamshid; Pezacki, John Paul Bioorganic Chemistry, 2007 , vol. 35, # 3 p. 284 - 293

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被引用次数: 10

摘要

A recombinant VH single-domain antibody recognizing staphylococcal protein A was functionalized on reactive lysine residues with N-hydroxysuccimidyl-activated 4- cyanobenzoate. Surface plasmon resonance analysis of antibody-antigen binding revealed that modified and unmodified antibodies bound protein A with similar affinities. Raman imaging of the modified antibodies indicated that the benzonitrile group provides ...