Novel probes showing specific fluorescence enhancement on binding to a hexahistidine tag

M Kamoto, N Umezawa, N Kato…

文献索引：Kamoto, Mie; Umezawa, Naoki; Kato, Nobuki; Higuchi, Tsunehiko Chemistry - A European Journal, 2008 , vol. 14, # 26 p. 8004 - 8012

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被引用次数: 24

摘要

Abstract The introduction of hexahistidine (His tag) is widely used as a tool for affinity purification of recombinant proteins, since the His tag binds selectively to nickel– nitrilotriacetic acid (Ni 2+–NTA) complex. To develop efficient “turn-on” fluorescent probes for His-tagged proteins, we adopted a fluorophore displacement strategy, that is, we designed probes in which a hydroxycoumarin fluorophore is joined via a linker to a metal– ...