Abstract The introduction of hexahistidine (His tag) is widely used as a tool for affinity purification of recombinant proteins, since the His tag binds selectively to nickel– nitrilotriacetic acid (Ni 2+–NTA) complex. To develop efficient “turn-on” fluorescent probes for His-tagged proteins, we adopted a fluorophore displacement strategy, that is, we designed probes in which a hydroxycoumarin fluorophore is joined via a linker to a metal– ...
[Black, Michael; Cadogan; McNab, Hamish; MacPherson, Andrew D.; Roddam, V. Peter; Smith, Carol; Swenson, Helen R. Journal of the Chemical Society - Perkin Transactions 1, 1997 , # 17 p. 2483 - 2493]
