Journal of Molecular Catalysis B: Enzymatic

Amino ester hydrolase from Xanthomonas campestris pv. campestris, ATCC 33913 for enzymatic synthesis of ampicillin

JK Blum, AS Bommarius

文献索引：Blum, Janna K.; Bommarius, Andreas S. Journal of Molecular Catalysis B: Enzymatic, 2010 , vol. 67, # 1-2 p. 21 - 28

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被引用次数: 23

摘要

α-Amino ester hydrolases (AEH) are a small class of proteins, which are highly specific for hydrolysis or synthesis of α-amino containing amides and esters including β-lactam antibiotics such as ampicillin, amoxicillin, and cephalexin. A BLAST search revealed the sequence of a putative glutaryl 7-aminocephalosporanic acid (GL-7-ACA) acylase 93% identical to a known AEH from Xanthomonas citri. The gene, termed gaa, was cloned from ...