Understanding protease catalysed solid phase peptide synthesis

RV Ulijn, N Bisek, PJ Halling, SL Flitsch

文献索引：Ulijn, Rein V.; Bisek, Nicola; Halling, Peter J.; Flitsch, Sabine L. Organic and Biomolecular Chemistry, 2003 , vol. 1, # 8 p. 1277 - 1281

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被引用次数: 25

摘要

A protease (thermolysin) was used to directly synthesise a number of dipeptides from soluble Fmoc-amino acids onto a solid support (PEGA1900) in bulk aqueous media, often in very good yields. This shift in equilibrium toward synthesis is remarkable because for soluble dipeptides in aqueous solution hydrolysis rather than synthesis is observed. Three possible reasons for the equilibrium shift were considered:(i) using a solid support makes ...