Enzymes in organic synthesis: use of subtilisin and a highly stable mutant derived from multiple site-specific mutations

CH Wong, ST Chen, WJ Hennen, JA Bibbs…

文献索引：Wong; Chen; Hennen; Bibbs; Wang; L iu; Pantoliano; Whitlow; Bryan Journal of the American Chemical Society, 1990 , vol. 112, # 3 p. 945 - 953

全文：HTML全文

被引用次数: 173

摘要

Abstract: A subtilisin mutant (subtilisin 8350) derived from subtilisin BPN'via six site-specific mutations (MetSOPhe, Gly169Ala, Asn76Asp, Gln206Cys, Tyr217Lys, and Asn218Ser) was found to be 100 times more stable than the wild-type enzyme in aqueous solution at room temperature and 50 times more stable than the wild type in anhydrous dimethylformamide. Kinetic studies using ester, thio ester, and amide substrates, and the transition-state ...