From a strain of Actinomadura madurae, a new siderophore was isolated. Maduraferrin is the Fe complex of an oligopeptide composed of salicylic acid, fl-alanine, glycine, L-serine, Nd-hydroxy-N"-methyl-L-ornithine, and~-hexahydropyridazine-3-carboxylic acid. The complexing centers are a salicylamide moiety, a hydroxamic-acid group and an acid hydrazide group.