Journal of the American Chemical Society

Manipulating the stereoselectivity of limonene epoxide hydrolase by directed evolution based on iterative saturation mutagenesis

H Zheng, MT Reetz

文献索引：Zheng, Huabao; Reetz, Manfred T. Journal of the American Chemical Society, 2010 , vol. 132, # 44 p. 15744 - 15751

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被引用次数: 57

摘要

Limonene epoxide hydrolase from Rhodococcus erythropolis DCL 14 (LEH) is known to be an exceptional epoxide hydrolase (EH) because it has an unusual secondary structure and catalyzes the hydrolysis of epoxides by a rare one-step mechanism in contrast to the usual two-step sequence. From a synthetic organic viewpoint it is unfortunate that LEH shows acceptable stereoselectivity essentially only in the hydrolysis of the natural substrate ...