Tetrahedron: Asymmetry

Rapid screening of hydrolases for the enantioselective conversion of 'difficult-to-resolve'substrates

M Baumann, BH Hauer, UT Bornscheuer

文献索引：Baumann, Markus; Hauer, Bernhard H.; Bornscheuer, Uwe T. Tetrahedron Asymmetry, 2000 , vol. 11, # 23 p. 4781 - 4790

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被引用次数: 75

摘要

Hydrolases showing high enantioselectivity towards three racemic alcohols (1-methoxy-2- propanol, 3-hydroxy-tetrahydrofuran, 3-butyn-2-ol) and pantolactone were identified by a step-wise screening procedure. Initially, those biocatalysts, which exhibited hydrolytic activity towards the corresponding acetates or butyrates, were selected out of> 100 enzymes. Here, rapid screening was performed in a pH-indicator, based format in ...