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548-62-9

548-62-9 structure
548-62-9 structure
  • Name: Crystal Violet
  • Chemical Name: crystal violet
  • CAS Number: 548-62-9
  • Molecular Formula: C25H30ClN3
  • Molecular Weight: 407.979
  • Catalog: Dyes and pigments dye Basic dye
  • Create Date: 2018-06-01 08:00:00
  • Modify Date: 2024-01-02 14:29:15
  • Crystal violet is a triarylmethane dye.Target: OthersCrystal Violet is a component of Gram staining that allows one to recognize the difference between gram-positive and gram-negative bacteria with differential staining. The compound helps to access bacterial contamination of tissue culture samples. The staining response is due to the difference in the chemical and structural composition of the cell walls in different bacteria. Crystal Violet can also be used to test the different species of archaea and the cytostatic/cytotoxic effects on tumor cell lines [1-3].
Name crystal violet
Synonyms Crystal violet
Basic Violet 3
EINECS 208-953-6
Methyl violet
MFCD00011750
Description Crystal violet is a triarylmethane dye.Target: OthersCrystal Violet is a component of Gram staining that allows one to recognize the difference between gram-positive and gram-negative bacteria with differential staining. The compound helps to access bacterial contamination of tissue culture samples. The staining response is due to the difference in the chemical and structural composition of the cell walls in different bacteria. Crystal Violet can also be used to test the different species of archaea and the cytostatic/cytotoxic effects on tumor cell lines [1-3].
Related Catalog
References

[1]. Beveridge, T.J. and S. Schultze-Lam, The response of selected members of the archaea to the gram stain. Microbiology, 1996. 142 ( Pt 10): p. 2887-95.

[2]. Coico, R., Gram staining. Curr Protoc Microbiol, 2005. Appendix 3: p. Appendix 3C.

[3]. Bil, J., et al., Statins potentiate cytostatic/cytotoxic activity of sorafenib but not sunitinib against tumor cell lines in vitro. Cancer Lett, 2010. 288(1): p. 57-67.
Density 1.19 g/cm3 (20ºC)
Melting Point 215ºC
Molecular Formula C25H30ClN3
Molecular Weight 407.979
Flash Point 40ºC
Exact Mass 407.212830
PSA 9.49000
LogP 1.46360

CHEMICAL IDENTIFICATION

RTECS NUMBER :
BO9000000
CHEMICAL NAME :
Ammonium, (4-(bis(p-(dimethylamino)phenyl)methylene)-2,5-cycloh exadien-1-ylidene) dimethyl-, chloride
CAS REGISTRY NUMBER :
548-62-9
LAST UPDATED :
199710
DATA ITEMS CITED :
48
MOLECULAR FORMULA :
C25-H30-N3.Cl
MOLECULAR WEIGHT :
408.03
WISWESSER LINE NOTATION :
L6Y DYJ AUYR DN1&1&R DN1&1& DUK1&1 &Q &G

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :
Standard Draize test
ROUTE OF EXPOSURE :
Administration onto the skin
SPECIES OBSERVED :
Human
TYPE OF TEST :
Standard Draize test
ROUTE OF EXPOSURE :
Administration onto the skin
SPECIES OBSERVED :
Human
TYPE OF TEST :
Standard Draize test
ROUTE OF EXPOSURE :
Administration onto the skin
SPECIES OBSERVED :
Rodent - guinea pig
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
420 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intraperitoneal
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
8900 ug/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
96 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intraperitoneal
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
5100 ug/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
20 mg/kg
TOXIC EFFECTS :
Lungs, Thorax, or Respiration - acute pulmonary edema
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Mammal - dog
DOSE/DURATION :
1 gm/kg
TOXIC EFFECTS :
Behavioral - food intake (animal) Gastrointestinal - nausea or vomiting Nutritional and Gross Metabolic - weight loss or decreased weight gain
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Mammal - cat
DOSE/DURATION :
100 mg/kg
TOXIC EFFECTS :
Gastrointestinal - hypermotility, diarrhea Gastrointestinal - nausea or vomiting Nutritional and Gross Metabolic - weight loss or decreased weight gain
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rabbit
DOSE/DURATION :
150 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intraperitoneal
SPECIES OBSERVED :
Rodent - rabbit
DOSE/DURATION :
5 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intraduodenal
SPECIES OBSERVED :
Rodent - rabbit
DOSE/DURATION :
160 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - guinea pig
DOSE/DURATION :
100 mg/kg
TOXIC EFFECTS :
Behavioral - ataxia
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Intraperitoneal
SPECIES OBSERVED :
Rodent - guinea pig
DOSE/DURATION :
10 mg/kg
TOXIC EFFECTS :
Lungs, Thorax, or Respiration - acute pulmonary edema
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Intraperitoneal
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
4 mg/kg/2D-I
TOXIC EFFECTS :
Gastrointestinal - peritonitis Related to Chronic Data - death
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
10950 mg/kg/2Y-C
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria Endocrine - thyroid tumors
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
25750 mg/kg/2Y-C
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria Kidney, Ureter, Bladder - tumors Reproductive - Tumorigenic effects - ovarian tumors
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
DOSE :
100 mg/kg
SEX/DURATION :
female 6-15 day(s) after conception
TOXIC EFFECTS :
Reproductive - Specific Developmental Abnormalities - musculoskeletal system Reproductive - Specific Developmental Abnormalities - urogenital system
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
DOSE :
40 mg/kg
SEX/DURATION :
female 6-15 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - post-implantation mortality (e.g. dead and/or resorbed implants per total number of implants)
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
DOSE :
100 mg/kg
SEX/DURATION :
female 6-15 day(s) after conception
TOXIC EFFECTS :
Reproductive - Specific Developmental Abnormalities - other developmental abnormalities
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
DOSE :
7 mg/kg
SEX/DURATION :
female 6-19 day(s) after conception
TOXIC EFFECTS :
Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death, e.g., stunted fetus)
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
DOSE :
7 mg/kg
SEX/DURATION :
female 6-19 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - post-implantation mortality (e.g. dead and/or resorbed implants per total number of implants) Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death, e.g., stunted fetus)

MUTATION DATA

TYPE OF TEST :
Cytogenetic analysis
TEST SYSTEM :
Non-mammalian species Cells - not otherwise specified
DOSE/DURATION :
500 ug/L
REFERENCE :
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 58,269,1978 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M1517 No. of Facilities: 9858 (estimated) No. of Industries: 58 No. of Occupations: 52 No. of Employees: 79824 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M1517 No. of Facilities: 3280 (estimated) No. of Industries: 21 No. of Occupations: 34 No. of Employees: 75632 (estimated) No. of Female Employees: 49319 (estimated)
Symbol GHS02 GHS07 GHS08 GHS09
GHS02, GHS07, GHS08, GHS09
Signal Word Warning
Hazard Statements H226-H319-H351-H411
Precautionary Statements P273-P281-P305 + P351 + P338
Personal Protective Equipment Eyeshields;Faceshields;full-face respirator (US);Gloves;multi-purpose combination respirator cartridge (US);type ABEK (EN14387) respirator filter
Hazard Codes T:Toxic
Risk Phrases R22;R41;R45;R50/53
Safety Phrases S45-S53-S60-S61
RIDADR UN 3077
WGK Germany 3
RTECS BO9000000
Packaging Group III
Hazard Class 9
HS Code 32041300
HS Code 32041300

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