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5-FAM SE

Names

[ CAS No. ]:
92557-80-7

[ Name ]:
5-FAM SE

[Synonym ]:
MFCD00077322
1-{[(3',6'-Dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthen]-5-yl)carbonyl]oxy}-2,5-pyrrolidinedione
5-Carboxyfluorescein N-succinimidyl ester
2,5-Pyrrolidinedione, 1-[[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]oxy]-
5-FAM SE

Biological Activity

[Description]:

5-FAM SE is a single isomer, it is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has also been used to prepare various small fluorescent molecules.

[Related Catalog]:

Signaling Pathways >> Others >> Others
Research Areas >> Others
Dye Reagents

[Related Small Molecules]

Captisol | Cyclosporin A | H2DCFDA | 0MPTP hydrochloride | GW4869 | Etomoxir | TD139 | Mitoquinone mesylate | GSK2795039 | JC-1 | BAPTA-AM | AP 20187 | Setanaxib (GKT137831) | D-Luciferin | Crotaline

Chemical & Physical Properties

[ Density]:
1.7±0.1 g/cm3

[ Boiling Point ]:
778.7±70.0 °C at 760 mmHg

[ Melting Point ]:
>300°C (dec.)

[ Molecular Formula ]:
C25H15NO9

[ Molecular Weight ]:
473.388

[ Flash Point ]:
424.7±35.7 °C

[ Exact Mass ]:
473.074677

[ PSA ]:
139.67000

[ LogP ]:
0.71

[ Vapour Pressure ]:
0.0±2.8 mmHg at 25°C

[ Index of Refraction ]:
1.790

[ Storage condition ]:
−20°C

MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xi

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

Articles

A DNA-based molecular probe for optically reporting cellular traction forces.

Nature Methods 11(12) , 1229-32, (2014)

We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring defo...

DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments.

Nucleic Acids Res. 20 , 2471, (1992)

The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP...

Nonradioactive labeling of RNA.

Anal. Biochem. 233 , 124, (1996)

A modified ribodinucleotide (named pSEEp) has been synthesized using commercially available components on a DNA synthesizer. The presence of a 3'-terminal primary amino group permits its coupling to a...


More Articles


Related Compounds

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