6-Carboxyfluorescein Diacetate

Names

[ Name ]:
6-Carboxyfluorescein Diacetate

[Synonym ]:
6-FAM DA
6-CFDA 6-Carboxy-di-O-acetylfluorescein
6-CFDA
MFCD00036871
6-Carboxyfluorescein diacetate
3',6'-Diacetoxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-6-carboxylic acid
6-Carboxy-di-O-acetylfluorescein,6-CFDA
5,6-diacetyl FAM-SE
6-CARBOXY-DI-O-ACETYLFLUORESCEIN
Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid, 3',6'-bis(acetyloxy)-3-oxo-
3',6'-Diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylic acid
6-FAM DIACETATE
6-Carboxyfluorescein diacetate,6-CFDA,6-Carboxy-di-O-acetylfluorescein
6-CARBOXYFLUORESCEINE DIACETATE

Biological Activity

[Description]:

6-CFDA is fluorescent polyanionic probe, and its derivatives such as 2′,7′-bis (2-carboxyethyl)-5(6)-carboxyfluorescein are used to measure changes in intracellular pH (pHi) and processes such as dendrimer aggregation and absorption.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Research Areas >> Others

Dye Reagents

[References]

[1]. Babcock DF. Examination of the intracellular ionic environment and of ionophore action by null point measurements employing the fluorescein chromophore. J Biol Chem. 1983 May 25;258(10):6380-9.

[2]. Graber ML, et al. Characteristics of fluoroprobes for measuring intracellular pH. Anal Biochem. 1986 Jul;156(1):202-12.

[3]. Bonizzoni M, et al. PAMAM dendrimer-induced aggregation of 5(6)-carboxyfluorescein. J Org Chem. 2012 Feb 3;77(3):1258-66.

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
1.6±0.1 g/cm3

[ Boiling Point ]:
701.6±60.0 °C at 760 mmHg

[ Melting Point ]:
152–153°C

[ Molecular Formula ]:
C₂₅H₁₆O₉

[ Molecular Weight ]:
460.389

[ Flash Point ]:
245.1±26.4 °C

[ Exact Mass ]:
460.079437

[ PSA ]:
125.43000

[ LogP ]:
2.45

[ Appearance of Characters ]:
Light yellow or off-white solid

[ Vapour Pressure ]:
0.0±2.3 mmHg at 25°C

[ Index of Refraction ]:
1.702

[ Storage condition ]:
2-8°C

[ Water Solubility ]:
DMSO: soluble

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xi

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ HS Code ]:
2932999099

Synthetic Route

Click to get detail synthetic route

Precursor & DownStream

Precursor

DownStream

Customs

[ HS Code ]: 2932999099

[ Summary ]:
2932999099. other heterocyclic compounds with oxygen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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