Adenylyl Imidodiphosphate

AMP-PNP (Adenylyl imidodiphosphate) is a non-hydrolysable ATP analogue[1].

Research Areas >> Others

Signaling Pathways >> Others >> Others

AMP-PNP binding induces an inward rotation and shift of the transmembrane helices of LptFG and LptC to tighten the cavity, with the closure of two lateral gates, to eventually expel LPS into the bridge[1]. AMP-PNP significantly inhibits the ATPase activity of sfLptB2FGC, which was used to mimic and lock an ATP-bound state of sfLptB2FGC[1].

[1]. Tang X, et al. Cryo-EM structures of lipopolysaccharide transporter LptB2FGC in lipopolysaccharide or AMP-PNP-bound states reveal its transport mechanism. Nat Commun. 2019 Sep 13;10(1):4175.