β-半乳糖苷酶

β-半乳糖苷酶用途

β-半乳糖苷酶是一种糖苷水解酶,可水解半乳糖与其有机部分之间形成的β-糖苷键。在大肠杆菌中,lacZ基因是β-半乳糖苷酶的结构基因,可作为诱导系统lac操纵子的一部分。β-半乳糖苷酶可水解乳糖形成葡萄糖和半乳糖,并进入糖酵解；它还可以催化乳糖转半乳糖基化为异乳糖；异乳糖可以裂解成单糖[1]。

β-半乳糖苷酶名称

[ CAS 号 ]:
9031-11-2

[ 中文名 ]:
beta-半乳糖苷酶

[ 英文名 ]:
Native Aspergillus oryzae β-Galactosidase

[英文别名 ]:

beta-Galactosidase
2-[2-(4-Heptylphenyl)ethyl]-6-hydroxybenzoic acid
Native Aspergillus oryzae β-Galactosidase

β-半乳糖苷酶生物活性

[ 描述 ]:

[ 相关类别 ]:

研究领域 >> 其他

[参考文献]

[1]. Douglas H Juers, et al. LacZ β-galactosidase: structure and function of an enzyme of historical and molecular biological importance. Protein Sci. 2012 Dec;21(12):1792-807.

β-半乳糖苷酶物理化学性质

[ 密度 ]:
1.1±0.1 g/cm3

[ 沸点 ]:
477.8±33.0 °C at 760 mmHg

[ 分子式 ]:
C₂₂H₂₈O₃

[ 分子量 ]:
340.456

[ 闪点 ]:
256.9±21.9 °C

[ 精确质量 ]:
340.203857

[ LogP ]:
8.19

[ 外观性状 ]:
powder | slightly yellow

[ 蒸汽压 ]:
0.0±1.3 mmHg at 25°C

[ 折射率 ]:
1.572

[ 储存条件 ]:
2-8°C

[ 水溶解性 ]:
1% acetic acid: 1 mg/mL | Soluble in water

β-半乳糖苷酶安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危害码 (欧洲) ]:
Xn

[ 风险声明 (欧洲) ]:
22

[ 安全声明 (欧洲) ]:
24/25

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

[ RTECS号 ]:
LW5840000

β-半乳糖苷酶制备

1.由制干酪时所得的乳清，在pH值4.5和温度85～105℃下加热凝固，在凝固物中加入0.1%的氨水，接入酵母菌种(如脆壁酵母，Saccharomyces fragilis)在30℃下通气培养，收集酵母，用温水洗净后在-18℃下急速冷却，使其中所含的其他酶类失活，然后用酵母量1.5～3.0倍的乙醇进行处理。

β-半乳糖苷酶文献

Initial characterization of the human central proteome.

BMC Syst. Biol. 5 , 17, (2011)

On the basis of large proteomics datasets measured from seven human cell lines we consider their intersection as an approximation of the human central proteome, which is the set of proteins ubiquitous...

Complete sequencing and characterization of 21,243 full-length human cDNAs.

Nat. Genet. 36 , 40-5, (2004)

As a base for human transcriptome and functional genomics, we created the "full-length long Japan" (FLJ) collection of sequenced human cDNAs. We determined the entire sequence of 21,243 selected clone...

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

Genome Res. 14 , 2121-7, (2004)

The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for...

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