外切核酸酶III_MSDS_结构式_性质_CAS号【9037-44-9】

外切核酸酶III结构式	常用名	外切核酸酶III	英文名	ExonucleaseIII
CAS号	9037-44-9	分子量	N/A
密度	N/A	沸点	N/A
分子式	N/A	熔点	N/A
MSDS	中文版美版	闪点	N/A

中文名	外切核酸酶III
英文名	ExonucleaseIII
英文别名	更多

储存条件	-20°C密闭，避光，通风干燥处
稳定性	Biochem/physiol Actions A 3′→5′ exonuclease which catalyzes the removal of mononucleotides from the 3′-end of dsDNA. The enzyme also has apurinic and apyrimidinic endonuclease, 3′-DNA phosphatase, and RNase H activities. Activity is strongly dependent on temperature, salt concentration, and the ratio of enzyme to DNA; reaction conditions must be optimized for specific applications. Features and Benefits • Production of strand specific probes1 • Preparation of single stranded templates for dideoxy sequencing2 • Site directed mutagenesis3 Unit Definition One unit releases 1 nmole of acid-soluble nucleotides from sonicated calf thymus DNA in 30 min at 37 °C . Physical form Solution in 5 mM potassium phosphate pH 6.5, 200 mM KCl, 0.05 mM EDTA, 5 mM 2-mercaptoethanol, 200 μg/ml BSA, and 50% glycerol
更多	1. 性状：丙三醇缓冲溶液。 2. 密度（g/mL, 25 ℃ ）：未确定 3. 相对蒸汽密度（g/mL,空气=1）：未确定 4. 熔点（ºC）：未确定 5. 沸点（ºC,常压）：未确定 6. 沸点（ºC,5.2kPa）：未确定 7. 折射率：未确定 8. 闪点（ºC）：未确定 9. 比旋光度（º）：未确定 10. 自燃点或引燃温度（ºC）：未确定 11. 蒸气压（kPa,20ºC）：未确定 12. 饱和蒸气压（kPa,60ºC）：未确定 13. 燃烧热（KJ/mol）：未确定 14. 临界温度（ºC）：未确定 15. 临界压力（KPa）：未确定 16. 油水（辛醇/水）分配系数的对数值：未确定 17. 爆炸上限（%,V/V）：未确定 18. 爆炸下限（%,V/V）：未确定 19. 溶解性：溶于水。

储存条件

-20°C密闭，避光，通风干燥处

稳定性

Biochem/physiol Actions A 3′→5′ exonuclease which catalyzes the removal of mononucleotides from the 3′-end of dsDNA. The enzyme also has apurinic and apyrimidinic endonuclease, 3′-DNA phosphatase, and RNase H activities. Activity is strongly dependent on temperature, salt concentration, and the ratio of enzyme to DNA; reaction conditions must be optimized for specific applications.

Features and Benefits • Production of strand specific probes1

• Preparation of single stranded templates for dideoxy sequencing2

• Site directed mutagenesis3

Unit Definition One unit releases 1 nmole of acid-soluble nucleotides from sonicated calf thymus DNA in 30 min at 37 °C .

Physical form Solution in 5 mM potassium phosphate pH 6.5, 200 mM KCl, 0.05 mM EDTA, 5 mM 2-mercaptoethanol, 200 μg/ml BSA, and 50% glycerol

更多

1. 性状：丙三醇缓冲溶液。

2. 密度（g/mL, 25 ℃ ）：未确定

3. 相对蒸汽密度（g/mL,空气=1）：未确定

4. 熔点（ºC）：未确定

5. 沸点（ºC,常压）：未确定

6. 沸点（ºC,5.2kPa）：未确定

7. 折射率：未确定

8. 闪点（ºC）：未确定

9. 比旋光度（º）：未确定

10. 自燃点或引燃温度（ºC）：未确定

11. 蒸气压（kPa,20ºC）：未确定

12. 饱和蒸气压（kPa,60ºC）：未确定

13. 燃烧热（KJ/mol）：未确定

14. 临界温度（ºC）：未确定

15. 临界压力（KPa）：未确定

16. 油水（辛醇/水）分配系数的对数值：未确定

17. 爆炸上限（%,V/V）：未确定

18. 爆炸下限（%,V/V）：未确定

19. 溶解性：溶于水。

个人防护装备	Eyeshields;Gloves;half-mask respirator (US);multi-purpose combination respirator cartridge (US)
危险品运输编码	NONH for all modes of transport

Combined micrococcal nuclease and exonuclease III digestion reveals precise positions of the nucleosome core/linker junctions: implications for high-resolution nucleosome mapping.

J. Mol. Biol. 425(11) , 1946-60, (2013)

Micrococcal nuclease (MNase) is extensively used in genome-wide mapping of nucleosomes but its preference for AT-rich DNA leads to errors in establishing precise positions of nucleosomes. Here, we sho...

Graphene oxide-based biosensor for sensitive fluorescence detection of DNA based on exonuclease III-aided signal amplification.

Anal. Chim. Acta 727 , 67-70, (2012)

Based on the super fluorescence quenching efficiency of graphene oxide and exonuclease III aided signal amplification, we develop a facile, sensitive, rapid and cost-effective method for DNA detection...

Two-step, PCR-free telomerase detection by using exonuclease III-aided target recycling.

ChemBioChem. 12(18) , 2745-7, (2011)

We report the sensitive detection of telomerase activity by using exonuclease III-aided target recycling to amplify the signal produced by a chimeric LNA-DNA molecular beacon. We demonstrate the speci...

MFCD00163580

外切核酸酶III

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外切核酸酶III物理化学性质

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外切核酸酶III英文别名