| In Vitro |
MD13 degrades 91±5% and 71±7% MIF at 2 μM and 0.2 μM, respectively. MD13 induces MIF degradation through binding to E3 ligase cereblon[1]. MD13 (0-20 μM; 72 h) inhibits cell proliferation of A549 cancer cells[1]. MD13 (1-5 μM; 48 h) arrests cell cycle at G2/M phase in A549 cells[1]. MD13 (2 μM; 6-48 h) inhibits ERK signaling[1]. Western Blot Analysis[1] Cell Line: A549 cells Concentration: 0-20 μM Incubation Time: 12 h Result: Caused depletion of MIF protein. Western Blot Analysis[1] Cell Line: A549 cells Concentration: 2 μM Incubation Time: 6, 24 or 48 h Result: Inhibited ERK phosphorylation. Cell Proliferation Assay[1] Cell Line: A549 cells Concentration: 0-20 μM Incubation Time: 72 h Result: Inhibited the growth of A549 cells in a dose-dependent manner. The inhibitory effect reached about 50% inhibition of cell proliferation at a concentration of 20 µM. Cell Cycle Analysis[1] Cell Line: A549 cells Concentration: 1, 2, or 5 μM Incubation Time: 48 h Result: Dose-dependently induced cell cycle arrest at the G2/M phase.
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