2758431-97-7
2758431-97-7 structure
- Name: MD13
- Chemical Name: MD13
- CAS Number: 2758431-97-7
- Molecular Formula: C35H35N5O8
- Molecular Weight: 653.68
- Catalog: Signaling Pathways PROTAC PROTAC
- Create Date: 2022-10-11 03:32:41
- Modify Date: 2023-05-12 14:16:50
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MD13 is a macrophage migration inhibitory factor (MIF)-directed PROTAC with a Ki of 71 nM. MD13 can be used for cancer research[1].
| Name | MD13 |
|---|
| Description | MD13 is a macrophage migration inhibitory factor (MIF)-directed PROTAC with a Ki of 71 nM. MD13 can be used for cancer research[1]. |
|---|---|
| Related Catalog | |
| Target |
Ki: 71 nM (MIF)[1] |
| In Vitro | MD13 degrades 91±5% and 71±7% MIF at 2 μM and 0.2 μM, respectively. MD13 induces MIF degradation through binding to E3 ligase cereblon[1]. MD13 (0-20 μM; 72 h) inhibits cell proliferation of A549 cancer cells[1]. MD13 (1-5 μM; 48 h) arrests cell cycle at G2/M phase in A549 cells[1]. MD13 (2 μM; 6-48 h) inhibits ERK signaling[1]. Western Blot Analysis[1] Cell Line: A549 cells Concentration: 0-20 μM Incubation Time: 12 h Result: Caused depletion of MIF protein. Western Blot Analysis[1] Cell Line: A549 cells Concentration: 2 μM Incubation Time: 6, 24 or 48 h Result: Inhibited ERK phosphorylation. Cell Proliferation Assay[1] Cell Line: A549 cells Concentration: 0-20 μM Incubation Time: 72 h Result: Inhibited the growth of A549 cells in a dose-dependent manner. The inhibitory effect reached about 50% inhibition of cell proliferation at a concentration of 20 µM. Cell Cycle Analysis[1] Cell Line: A549 cells Concentration: 1, 2, or 5 μM Incubation Time: 48 h Result: Dose-dependently induced cell cycle arrest at the G2/M phase. |
| References |
| Molecular Formula | C35H35N5O8 |
|---|---|
| Molecular Weight | 653.68 |