| In Vitro |
FLT3-IN-14 (compound 9c) (0-10 μM; 24 hours) inhibits the proliferation of tested twelve haematological cell lines with IC50s of 0.011-1.582 μM[1]. FLT3-IN-14 (0-10 μM; 72 hours) exhibits low toxicity, with GI50 greater than 10 μM, in resting lymphocytes[1]. FLT3-IN-14 (1-50 nM; 24 and 48 hours) accumulates annexin-V positive cells in a concentration and time-dependent manner[1]. FLT3-IN-14 (25-100 nM; 24 and 48 hours) induces a significant G1 arrest in both cell lines[1]. FLT3-IN-14 (1-50 nM; 24 hours) induces the dephosphorylation of FLT3[1]. Cell Proliferation Assay Cell Line: MOLT-4 , HL-60, KG-1, KG-1a, MOLM-13, MV4-11, NOMO-1, OCI-AML2, PL-21, THP-1, K-562, KCL-22[1] Concentration: 0-10 μM Incubation Time: 24 hours Result: Inhibited the proliferation of these twelve haematological cell lines with IC50s of 0.011-1.582 μM. Cell Cytotoxicity Assay Cell Line: PBL[1] Concentration: 0-10 μM Incubation Time: 72 hours Result: Exhibited low toxicity, with GI50 greater than 10 μM, in resting lymphocytes. Apoptosis Analysis Cell Line: MV4-11[1] Concentration: 1, 10 and 50 nM Incubation Time: 24 and 48 hours Result: Accumulated annexin-V positive cells in a concentration and time-dependent manner. Cell Cycle Analysis Cell Line: MOLM-13 and MV-14[1] Concentration: 25, 50, 75 and 100 nM Incubation Time: 24 and 48 hours Result: Induced a significant G1 arrest in both cell lines. Western Blot Analysis Cell Line: MV-14[1] Concentration: 1, 10 and 50 nM Incubation Time: 24 hours Result: Induced the dephosphorylation of FLT3.
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