DMA (hydrochloride)

Modify Date: 2024-01-06 22:44:29

DMA (hydrochloride) structure	Common Name	DMA (hydrochloride)
	CAS Number	2095832-33-8	Molecular Weight	577.93
	Density	N/A	Boiling Point	N/A
	Molecular Formula	C₂₇H₃₁Cl₃N₆O₂	Melting Point	N/A
	MSDS	N/A	Flash Point	N/A

Use of DMA (hydrochloride)

DMA trihydrochloride is a fluorescent compound (λex=340 nm, λem=478 nm).

Name	DMA trihydrochloride
Synonym	More Synonyms

Description	DMA trihydrochloride is a fluorescent compound (λex=340 nm, λem=478 nm).
Related Catalog	Signaling Pathways >> Others >> Others Research Areas >> Cancer Dye Reagents
Target	IC50: 3.4 μM (HeLa cell), 5.3 μM (MCF7 cell)[1]
In Vitro	The newly synthesized bisbenzimidazole derivatives DMA (6c) is evaluated for their cytotoxicity against human tumor cell lines, which are cervix carcinoma cell line (HeLa), breast carcinoma cell line (MCF7) and brain glioma cell line (U87) in comparison to Hoechst. In case of MCF7, the IC50 is observed at 5.3 μM for DMA. The IC50 determined in the case of HeLa is 3.4 μM for DMA[1].
Cell Assay	Various human tumor cells (U87, HeLa and MCF7) are maintained as monolayer at 37°C in 5% CO2 using DMEM medium. Approximately 3000-8000 cells/well are seeded in 96-well plates containing 200 μL of medium and incubated for 24 h. The culture medium is replaced by fresh medium containing 1, 10, 50, 100 μM of DMA (6c) and incubated for 24, 48 and 72 h. The cell viability is determined by the MTT assay. The light absorbance is measured using a microplate reader[1].
References	[1]. Singh M, et al. Synthesis and biological activity of novel inhibitors of topoisomerase I: 2-aryl-substituted 2-bis-1H-benzimidazoles. Eur J Med Chem. 2011 Feb;46(2):659-69.

Molecular Formula	C₂₇H₃₁Cl₃N₆O₂
Molecular Weight	577.93
Storage condition	-20℃

DMA (trihydrochloride)