| Description |
Cobomarsen (MRG-106) is an oligonucleotide inhibitor of miR-155. Cobomarsen inhibits multiple gene pathways associated with cell survival (including JAK/STAT, MAPK/ERK and PI3K/AKT). Cobomarsen can be used for the research of B-cell lymphoma[1][2].
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| Related Catalog |
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| In Vitro |
Cobomarsen (10 μM; 48-96 h) reduces cell proliferation and induces apoptosis in U2932, OCI-LY3, and RCK8 cells[2]. Cobomarsen (10 μM; 72 h) significantly increases the expression of the four direct targets in the cell lines that overexpress miR-155[1]. Cobomarsen (10 μM; 12 days) reduces cellular proliferation and induces apoptosis in MF and HTLV-1+ CTCL cells[1]. Cobomarsen (10-50 μM; 7 days) reduces phosphorylation of the downstream signalling proteins AKT, ERK1/2, and STAT-3 in primary human activated T cells or MF cell lines[1]. Cell Proliferation Assay[2] Cell Line: U2932, OCI-LY3 and RCK8 cells Concentration: 10 μM Incubation Time: 48, 72, 96 hours Result: Concentration-dependently inhibited cell phosphorylation. Apoptosis Analysis[2] Cell Line: U2932, OCI-LY3 and RCK8 cells Concentration: 10 μM Incubation Time: 96 hours Result: Triggered a significant induction of late apoptosis in all cell lines.
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| In Vivo |
Cobomarsen (1 mg/kg; i.v. on days 0, 2, 4, and 7) inhibits tumor growth in mice carrying U2932 cells xenografts[2]. Animal Model: Five-to-six weeks old female NSG mice injected with U2932 cells Dosage: 1 mg/kg Administration: I.v. on days 0, 2, 4, and 7 Result: Reduced tumor growth most significantly at day 7 and day 10.
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| References |
[1]. Seto AG, et, al. Cobomarsen, an oligonucleotide inhibitor of miR-155, co-ordinately regulates multiple survival pathways to reduce cellular proliferation and survival in cutaneous T-cell lymphoma. Br J Haematol. 2018 Nov;183(3):428-444. [2]. Anastasiadou E, et, al. Cobomarsen, an Oligonucleotide Inhibitor of miR-155, Slows DLBCL Tumor Cell Growth In Vitro and In Vivo. Clin Cancer Res. 2021 Feb 15;27(4):1139-1149.
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