| Description |
Vanicoside B is a phenylpropanoyl sucrose derivative, can be isolated from the herb Persicaria dissitiflora. Vanicoside B targets cyclin-dependent kinase 8 (CDK8) and exhibits anti-tumor activity. The potential mechanism is Vanicoside B blocks CDK8-mediated signaling pathways and decreases the expression of epithelial-mesenchymal transition proteins, so that it leads to cell cycle arrest and apoptosis[1][2].
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| Related Catalog |
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| Target |
CDK3
STAT3
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| In Vitro |
钒苷 B (2.5-20 μM; 72 h) 对三阴性乳腺癌 (TNBC) MDA-MB-231 和 HCC38 癌细胞具有抗增殖活性[1]。 钒苷 B (2.5-20 μM; 72 h, 14 d 和 72 h) 抑制 TNBC 细胞的细胞活力、集落形成，干扰细胞周期[1]。 钒苷 B (2.5-10 μM; 48 h) 降低了 p-STAT1，p-STAT3 和 p-S6 的蛋白水平，并通过调节 Skp2-p27 系列蛋白表达而诱导 TNBC 细胞凋亡[1]。 Western Blot Analysis[1] Cell Line: MDA-MB-231 cells and HCC38 cells Concentration: 0, 2.5, 5, 10 μM Incubation Time: 48 hours Result: Increased cleaved PARP, and p27 protein expressions, but decreased Skp2 protein level. Suppressed CDK8 target genes and the expression of EMT-associated proteins. Suppressed the expression of the cell proliferation marker Ki-67 in tumor tissues, also significantly suppressed the expressions of p-STAT1 (S727) and AXL. Cell Cycle Analysis[1] Cell Line: MDA-MB-231 cells and HCC38 cells Concentration: 0, 2.5, 5, 10 μM Incubation Time: 72 hours Result: Inhibited cell cycle at sub-G1 phase.
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| In Vivo |
钒苷 B (5 mg/kg 或 20 mg/kg; 腹腔注射; 每周 3 次, 共 4 周) 抑制 MDAMB-231 细胞移植的小鼠模型中的肿瘤生长[1]。 Animal Model: MDA-MB-231 cell-implanted xenograft mouse model[1] Dosage: 5 mg/kg, 20 mg/kg Administration: Intraperitoneal injection; 3 times per week over 4 weeks Result: Significantly reduced tumor volumes at 5 mg/kg and 20 mg/kg by 53.85% and 65.72%, respectively.
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| References |
[1]. Kim D, et al. Antitumor Activity of Vanicoside B Isolated from Persicaria dissitiflora by Targeting CDK8 in Triple-Negative Breast Cancer Cells. J Nat Prod. 2019 Nov 22;82(11):3140-3149. [2]. Takasaki M, et al. Cancer chemopreventive activity of phenylpropanoid esters of sucrose, vanicoside B and lapathoside A, from Polygonum lapathifolium. Cancer Lett. 2001 Nov 28;173(2):133-8.
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