3520-43-2
3520-43-2 structure
- Name: JC-1
- Chemical Name: 1,1',3,3'-tetraethyl-5,5',6,6'-tetrachloroimidacarbocyanine iodide
- CAS Number: 3520-43-2
- Molecular Formula: C25H27Cl4IN4
- Molecular Weight: 652.225
- Catalog: Dye Reagents
- Create Date: 2018-10-21 18:03:09
- Modify Date: 2024-01-13 20:17:11
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JC-1 is a fluorescent lipophilic carbocyanine dye used to measure mitochondrial membrane potential.
| Name | 1,1',3,3'-tetraethyl-5,5',6,6'-tetrachloroimidacarbocyanine iodide |
|---|---|
| Synonyms |
5,6-Dichloro-2-[(1E)-3-(5,6-dichloro-1,3-diethyl-1,3-dihydro-2H-benzimidazol-2-ylidene)-1-propen-1-yl]-1,3-diethyl-1H-3,1-benzimidazol-3-ium iodide
1H-3,1-Benzimidazolium, 5,6-dichloro-2-[(1E)-3-(5,6-dichloro-1,3-diethyl-1,3-dihydro-2H-benzimidazol-2-ylidene)-1-propen-1-yl]-1,3-diethyl-, iodide (1:1) 5,6-dichloro-2-[3-(5,6-dichloro-1,3-diethyl-2(3H)-benzimidazolylidene)-1-propenyl]-1,3-diethylbenzimidazolium iodide 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyanine iodide 5,5'6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine iodide 1,1',3,3'-tetraethyl-5,5'6,6'-tetrachlorobenzimidazolocarbocyanine iodide 5,6-dichloro-2-<1-<3-(5,6-dichloro-1,3-diethyl-2-benzimidazolylidene)propenyl>>-1,3-diethylbenzimidazolium iodide 1,3,1',3'-Tetraethyl-5,6,5',6'-tetrachloro-2,2'-imidacarbocyanine iodide 5,6-Dichloro-2-[(1E)-3-(5,6-dichloro-1,3-diethyl-1,3-dihydro-2H-benzimidazol-2-ylidene)prop-1-en-1-yl]-1,3-diethyl-1H-3,1-benzimidazol-3-ium iodide JC-1 5,5',6,6'-TETRACHLORO-1,1',3,3'-*TETRAETHYBENZIMIDA |
| Description | JC-1 is a fluorescent lipophilic carbocyanine dye used to measure mitochondrial membrane potential. |
|---|---|
| Related Catalog | |
| In Vitro | JC-1 (2.5 μM) exposed to murine L1210 lymphoblasts, can be detected the presence of both cytoplasmic JC-1 monomer and mitochondrial J-aggregates in these cells. JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers[1]. Fluorescent labeling of mitochondria with either JC-1 (1 μg/mL, 15 min), reveals that are distributed irregularly, resulting in regions of high and low mitochondrial content within astrocytes[2]. JC-1 has been shown to interact with α-synuclein at the acidic C-terminal region with a Kd of 2.6 μM. JC-1 itself does not accelerate the protein aggregation of α-synuclein in the absence of iron, insted, it decelerates the aggregation process by extending the lag phase approx[3]. JC-1 is avidly accumulated in sensitive K562 cells where it displays both a green cytoplasmic and red mitochondrial fluorescence. JC-1 is poorly accumulated in resistant K562 cells, which displays only a slight green fluorescence[4]. |
| References |
| Molecular Formula | C25H27Cl4IN4 |
|---|---|
| Molecular Weight | 652.225 |
| Exact Mass | 650.003418 |
| PSA | 18.67000 |
| LogP | 4.15720 |
| Storage condition | -20℃ |
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| RIDADR | NONH for all modes of transport |
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| Literature: Liebigs Annales, , # 1 p. 27 - 33 |
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| Literature: Liebigs Annales, , # 1 p. 27 - 33 |
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| Literature: Liebigs Annales, , # 1 p. 27 - 33 |
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| Literature: US2739149 , ; |
