323577-36-2

323577-36-2 structure

Name: Mca-(Ala7,Lys(Dnp)9)-Bradykinin trifluoroacetate salt
Chemical Name: (7-methoxycoumarin-4-yl)acetyl-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys-(2,4-dinitrophenyl)
CAS Number: 323577-36-2
Molecular Formula: C₆₆H₈₁N₁₅O₁₉
Molecular Weight: 1388.44000
Catalog: Research Areas Others
Create Date: 2017-12-03 17:51:00
Modify Date: 2024-01-11 16:03:58
Mca-(ala7,lys(dnp)9)-bradykinin is a sensitive fluorogenic substrate for ECE-1 (endothelin-converting enzyme-1). The incorporation of a (7-methoxycoumarin-4-yl)acetyl (Mca) fluorescent group and a 2,4-dinitrophenyl (Dnp) quenching group has resulted in a large fluorescence increase upon substrate cleavage[1].

Name	(7-methoxycoumarin-4-yl)acetyl-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys-(2,4-dinitrophenyl)
Synonyms	7-methoxycoumarin-4-yl-acetyl-RPPGFSAFK-2,4-dinitrophenyl McaBk2 MCA-ARG-PRO-PRO-GLY-PHE-SER-ALA-PHE-LYS(DNP)-OH Mca-(Ala7,Lys(Dnp)9)-Bradykinin

Description	Mca-(ala7,lys(dnp)9)-bradykinin is a sensitive fluorogenic substrate for ECE-1 (endothelin-converting enzyme-1). The incorporation of a (7-methoxycoumarin-4-yl)acetyl (Mca) fluorescent group and a 2,4-dinitrophenyl (Dnp) quenching group has resulted in a large fluorescence increase upon substrate cleavage[1].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Mca-(ala7,lys(dnp)9)-bradykinin is also a substrate for neprilysin, but is hydrolyzed 10-fold more efficiently by ECE-1, making this substrate selective for ECE-1[1].
References	[1]. Johnson GD, et al. Development of an internally quenched fluorescent substrate selective for endothelin-converting enzyme-1. Anal Biochem. 2000 Nov 1;286(1):112-8.