13860-38-3
13860-38-3 structure
- Name: N1-Methylpseudouridine
- Chemical Name: 1-methylpseudouridine
- CAS Number: 13860-38-3
- Molecular Formula: C10H14N2O6
- Molecular Weight: 258.22800
- Catalog: Research Areas Inflammation/Immunology
- Create Date: 2017-12-17 15:34:43
- Modify Date: 2024-01-10 00:00:53
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N1-methyl-pseudouridine (1-Methylpseudouridine), a methylpseudouridine, outperforms 5 mC and 5 mC/N1-methyl-pseudouridine in translation. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density[1].
| Name | 1-methylpseudouridine |
|---|---|
| Synonyms |
1-Methylpseudouridine
5-[(2S,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-1-methylpyrimidine-2,4-dione |
| Description | N1-methyl-pseudouridine (1-Methylpseudouridine), a methylpseudouridine, outperforms 5 mC and 5 mC/N1-methyl-pseudouridine in translation. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density[1]. |
|---|---|
| Related Catalog | |
| In Vitro | Incorporation of N1-methyl-pseudouridine into mRNA modifies mRNAs produced higher amounts of luc than the standard Luc mRNA in HEK293T cells. Incorporation of N1-methyl-pseudouridine nucleoside modification in both Luc and GFP mRNA enhances the initiation step of translation, in part by suppressing eIF2α phosphorylation. In addition, polysome formation and growth on the NN1-methyl-pseudouridine-containing Luc mRNA is enhanced due to the reduction of elongation rate. In all the in vitro translation systems, incorporation of N1-methyl-pseudouridine in Luc and GFP mRNAs dramatically enhanced translation. The N1-methyl-pseudouridine-Luc mRNA is associated with heavier polysomes than Luc mRNA[1]. |
| In Vivo | N1-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice[2]. N1-methyl-pseudouridine (1-Methylpseudouridine) (20 μg; I.m. or i.d. routes for 21 days) and m5C/ N1-methyl-pseudouridine-modified mRNA respectively have a higher translational capacity than Ψ and m5C/Ψ-modified mRNA in vivo[2]. Animal Model: 7-week-old Balb/c mice[1] Dosage: 20 μg Administration: I.m. or i.d. routes for 21 days Result: had a higher translational capacity. |
| References |
| Density | 1.576g/cm3 |
|---|---|
| Melting Point | 189 °C |
| Molecular Formula | C10H14N2O6 |
| Molecular Weight | 258.22800 |
| Exact Mass | 258.08500 |
| PSA | 124.78000 |
| Index of Refraction | 1.618 |