210345-03-2

210345-03-2 structure

Name: Ac-Leu-Glu-His-Asp-AFC trifluoroacetate salt
Chemical Name: 4-[(2-acetamido-4-methylpentanoyl)amino]-5-[[1-[[3-carboxy-1-oxo-1-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]propan-2-yl]amino]-3-(1H-imidazol-5-yl)-1-oxopropan-2-yl]amino]-5-oxopentanoic acid
CAS Number: 210345-03-2
Molecular Formula: C₃₃H₃₈F₃N₇O₁₁
Molecular Weight: 765.690
Catalog: Signaling Pathways Apoptosis Caspase
Create Date: 2016-06-01 22:42:26
Modify Date: 2025-08-26 18:14:52
Ac-LEHD-AFC is a fluorescent substrate for caspase-9, can be used to assess the activity of caspase[1].

Name	4-[(2-acetamido-4-methylpentanoyl)amino]-5-[[1-[[3-carboxy-1-oxo-1-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]propan-2-yl]amino]-3-(1H-imidazol-5-yl)-1-oxopropan-2-yl]amino]-5-oxopentanoic acid
Synonyms	L-α-Asparagine, N-acetyl-L-leucyl-L-α-glutamyl-L-histidyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]- Acetyl-Leu-Glu-His-Asp-AFC Ac-LEHD-AFC AFC154 PASE-9 SUBSTRATE,FLUOROGENIC N-Acetyl-L-leucyl-L-α-glutamyl-L-histidyl-N-[2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl]-L-α-asparagine Ac-Leu-Glu-His-Asp-7-Amino-4-trifluoromethylcoumarin Ac-Leu-Glu-His-Asp-AFC

Description	Ac-LEHD-AFC is a fluorescent substrate for caspase-9, can be used to assess the activity of caspase[1].
Related Catalog	Research Areas >> Others Signaling Pathways >> Apoptosis >> Caspase
Target	Caspase-9[1]
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). The assessment of the caspase activity by fluorogenic substrate assay[1]: 1. Preparation of cell lysis buffer: 1 % Nonidet P-40, NaCl 200 mM, Tris/HCl 20 mM, pH 7.4, Leupeptin 10 mg/L, Aprotinin (HY-P0017) (0.27 kU/L) and 100 mM PMSF (HY-B0496) . 2. Incubate the lysis buffer (25 mg of total protein) with 50 mmol/L of AC-DEVD-AMC (HY-P1003) (caspase-3), AC-VEID-AFC (caspase-8) or AC-LEHD-AFC (caspase-9) in the buffer (HEPES 10 mM, pH 7.4, containing Mannitol 220 mM, Sucrose 68 mM, NaCl 2 mM, KH2PO4 2.5 mM, EGTA 0.5 mM, MgCl2 2 mM, Pyruvate 5 mM, PMSF 0.1 mM, and Dithiothreitol 1 mM). 3. Assess the caspase activity by measuring fluorescent 7-amino-4-methylcoumarin released for 1 h at a 2-min intervals by a spectrofluorometer.