132319-57-4

132319-57-4 structure

Name: Mag-Fura-2 tetrapotassium
Chemical Name: Furaptra Tetrapotassium Salt
CAS Number: 132319-57-4
Molecular Formula: C₁₈H₁₀K₄N₂O₁₁
Molecular Weight: 586.67200
Catalog: Research Areas Others
Create Date: 2017-03-23 10:43:46
Modify Date: 2024-01-12 08:50:49
Mag-Fura-2 tetrapotassium is a UV excitable rational fluorescent Mg2+/Ca2+ indicator (Ex=334-360 nm, Em=510 nm). Mag-Fura-2 tetrapotassium can be used for the determination of Mg2+ and Ca2+ concentrations[1][2].

Name	Furaptra Tetrapotassium Salt
Synonyms	Mag-Fura-2,tetrapotassium salt [Furaptra,tetrapotassium salt] Fura 2 magnesium-selective analog tetrapotassium salt Mag-fura-2 tetrapotassium salt

Description	Mag-Fura-2 tetrapotassium is a UV excitable rational fluorescent Mg2+/Ca2+ indicator (Ex=334-360 nm, Em=510 nm). Mag-Fura-2 tetrapotassium can be used for the determination of Mg2+ and Ca2+ concentrations[1][2].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Monitoring of Ca2+ release[1]: 1. Incubate dispersed parotid acinar cells with 8 µM Mag-fura-2 tetrapotassium for 30 min at 37℃. (acinar cells for example). 2. Wash cells twice with fresh HBSS-H without BSA. 3. Precoate cell adhesive Cell-Tak on sample chambers. 4. Transfer the dye-loaded cells to the chambers and attach to the bottom. 5. Mounte the sample chambers on the stage of an inverted microscope (equipped with a 40 × objective), wash with BSA-free HBSS-H and then with Mg2+/ATP-free ICM. 6. Incubate acinar cells with Mg2+/ATP-free ICM containing 50 µg/mL saponin for 3-5 min at room temperature. 7. Wash the cells with ICM containing Mg2+ and ATP, and incubate in the complete ICM for at least 5 min to allow complete filling of the intracellular Ca2+ stores. 8. Alternately excite permeabilised cells, capture and digitise fluorescence emission at 510 nm by a digital imaging system (record the 344 nm/360 nm ratio every 20 s). Note: ICM (intracellular-like medium) containing 125 mM KCl, 19 mM NaCl, 10 mM HEPES (pH 7.3 with KOH), 3 mM ATP, 1.4 mM MgCl2, 0.33 mM CaCl2, and 1 mM EGTA.
References	[1]. Tojyo Y, et al. Monitoring of Ca2+ release from intracellular stores in permeabilized rat parotid acinar cells using the fluorescent indicators Mag-fura-2 and calcium green C18. Biochem Biophys Res Commun. 1997 Nov 7;240(1):189-95. [2]. Dai LJ, et al. Intracellular Mg2+ and magnesium depletion in isolated renal thick ascending limb cells. J Clin Invest. 1991 Oct;88(4):1255-64.

Boiling Point	803.3ºC at 760mmHg
Molecular Formula	C₁₈H₁₀K₄N₂O₁₁
Molecular Weight	586.67200
Flash Point	439.6ºC
Exact Mass	585.88300
PSA	212.16000
Vapour Pressure	3.08E-27mmHg at 25°C

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
RIDADR	NONH for all modes of transport

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