138777-25-0

138777-25-0 structure

Name: C12FDG
Chemical Name: N-[3-oxo-3',6'-bis[[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy]spiro[2-benzofuran-1,9'-xanthene]-5-yl]dodecanamide
CAS Number: 138777-25-0
Molecular Formula: C₄₄H₅₅NO₁₆
Molecular Weight: 853.90500
Catalog: Research Areas Others
Create Date: 2018-12-24 12:41:55
Modify Date: 2024-01-08 12:34:12
C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than FDG (HY-101895) for beta-galactosidase activity determinations in animal cells[1].

Name	N-[3-oxo-3',6'-bis[[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy]spiro[2-benzofuran-1,9'-xanthene]-5-yl]dodecanamide
Synonyms	5-Dodecanoylaminoflorescein di-b-D-galactopyranoside W0468

Description	C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than FDG (HY-101895) for beta-galactosidase activity determinations in animal cells[1].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Fluorescent senescence-associated β-galactosidase (SA-β-Gal) assay[2]: 1. Culture cells in 6-, 12- , 24-, or 96-well plates at a density of 5× 105 cells/mL overnight. Incubate the cells according to your normal protocol. 2. Wash cells by 200 μL of PBS once and fix with 100 μL of fixation solution (2% formaldehyde/0.2% glutaraldehyde in distilled water) at room temperature for 5 min. 3. Wash cells by 200 μL of PBS two times, and stain with 100 μL of 33 μM C12FDG (in PBS, pH=6.0) for 10 min, and with 200 μL of Hoechst solution (1 μg/mL Hoechst 33342 (HY-15559) in PBS, pH 6.0) for 10 min. 4. Image these cells by a 20× objective and 360-nm (Hoechst 33342) and 480-nm (C12FDG) excitation filters, and monitor through 460-nm and 535-nm emission filters, respectively.
References	[1]. Plovins A, et al. Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells. Appl Environ Microbiol. 1994 Dec;60(12):4638-41. [2]. Udono M, et al. Quantitative analysis of cellular senescence phenotypes using an imaging cytometer. Methods. 2012 Mar;56(3):383-8.

Molecular Formula	C₄₄H₅₅NO₁₆
Molecular Weight	853.90500
Exact Mass	853.35200
PSA	266.88000
LogP	3.12090
Storage condition	-20°C

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